The enabling legislation established “Route No. 45. From Route No. 49 in Salem, through Woodstown, Mullica Hill, Woodbury, to Route No. 47 in Brooklawn.” The only difference today is that the road in Brooklawn that NJ 45 ends at is US 130; that road, originally part of NJ 44, and NJ 45 ran together for less than half a mile to the intersection with NJ 47, which now terminates less than 500 feet from its original north end.
The north end of NJ 45 assumes the old Woodbury and Mantua Turnpike, which ran down to Mullica Hill, in Harrison Township. Below there, the road forks; the highway to Salem is NJ 45, while the road to Bridgeton was legislated as NJ 46 and is now NJ 77.
US 40 overlays NJ 45 for half a mile in Woodstown, while US 322 overlays it for half a mile in Mullica Hill.
| Mile | Street Name | Feature | ||
Road continues northward as  |
||||
| 28.51 | Northern terminus of , Westville Boro, Gloucester Co |
|||
| 28.51 | Broad St | |
merge | |
| 28.42 | Broad St | |
underpass | |
| 28.19 | Broad St | Railroad | at grade | Railroad |
| 28.01 | Broad St | Olive St |  |
Olive St |
| 27.80 | Broad St | Westville Boro, Gloucester Co West Deptford Twp, Gloucester Co |
Westville Boro, Gloucester Co Deptford Twp, Gloucester Co |
|
| 27.65 | Broad St |  |
underpass | |
| 27.47 | Broad St | Ramp from north to north |
underpass | |
| 27.25 | Broad St | Colonial Ave | |
|
| 27.02 | Broad St |  Hessian Ave |
|
|
| 26.93 | Broad St | West Deptford Twp, Gloucester Co Woodbury City, Gloucester Co |
Deptford Twp, Gloucester Co Woodbury City, Gloucester Co |
|
| 26.93 | Broad St | |
Park Ave |
|
| 26.79 | Broad St | |
Edith Ave | |
| 26.31 | Broad St |  Red Bank Ave |
|
Red Bank Ave |
| 26.21 | Broad St | Woodbury Creek | bridge | Woodbury Creek |
| 25.91 | Broad St | Cooper St | |
Cooper St, to  |
| 25.79 | Broad St | Centre St | |
Centre St |
| 25.69 | Broad St | Barber Ave | |
Barber Ave |
| 25.49 | |
Penn StSalem Ave |
|
Carpenter St |
| 25.35 | Mantua Ave | Railroad | at grade | Railroad |
| 25.30 | Mantua Ave | Twells Ave | |
Bellevue Ave |
| 24.93 | Mantua Ave | Railroad | at grade | Railroad |
| 24.93 | Mantua Ave | Woodbury City, Gloucester Co West Deptford Twp, Gloucester Co |
Woodbury City, Gloucester Co Woodbury Heights Boro, Gloucester Co |
|
| 24.90 | Mantua Ave | |
Evergreen Ave |
|
| 24.40 | Mantua Ave | Griscolm Ln | |
Elm Ave |
| 24.25 | Mantua Ave |   New Jersey Turnpike |
overpass | New Jersey Turnpike |
| 23.88 | Mantua Ave | Woodbury Heights Boro, Gloucester Co Deptford Twp, Gloucester Co |
||
| 23.88 | Mantua Ave | Riviera Dr | |
College Blvd |
| 23.34 | Mantua Ave |  Parkville Rd |
|
Parkville Rd, to Ogden Ave |
| 23.24 | Mantua Ave |  Ogden Ave |
Ogden Ave | |
| 22.76 | |
West Deptford Twp, Gloucester Co Mantua Twp, Gloucester Co |
Deptford Twp, Gloucester Co Mantua Twp, Gloucester Co |
|
| 22.76 | |
Mantua Creek | bridge | Mantua Creek |
| 22.69 | Bridgeton Pike | |
Main St ALT |
|
| 22.56 | Bridgeton Pike |  Berkley Rd |
|
Mantua Blvd |
| 22.27 | Bridgeton Pike | Branch Mantua Creek | culvert | Branch Mantua Creek |
| 22.09 | Bridgeton Pike | Shopping center entrance | |
Washington Ave |
| 21.73 | Bridgeton Pike |  Harrison Ave |
|
Mount Royal Rd |
| 21.60 | Bridgeton Pike | Jackson Rd |
||
| 20.82 | Bridgeton Pike | Edwards Run | bridge | Edwards Run |
| 19.45 | |
Mantua Twp, Gloucester Co Harrison Twp, Gloucester Co |
||
| 19.35 | Main St | Jefferson St |
||
| 19.17 | Main St |  Cohawkin Rd |
|
Cedar Rd |
| 19.00 | Main St | Breakneck Rd |
||
| 18.69 | Main St |  Tolfert Station Rd |
|
|
| 18.16 | Main St |   Swedesboro Rd |
|
|
| 17.78 | Main St | Mullica Hill Rd |
||
| 17.73 | Main St | Raccoon Creek | bridge | Raccoon Creek |
| 17.68 | Main St | Branch Raccoon Creek | culvert | Branch Raccoon Creek |
| 17.40 | Woodstown Rd | Bridgeton Pike |
||
| 16.42 | Woodstown Rd | South Branch Raccoon Creek | bridge | South Branch Raccoon Creek |
| 16.03 | Woodstown Rd | Harrison Twp, Gloucester Co South Harrison Twp, Gloucester Co |
||
| 15.65 | Woodstown Rd |  Swedesboro-Franklinville Rd |
|
Swedesboro-Franklinville Rd |
| 15.48 | Woodstown Rd | Mullica Hill Rd |
||
| 14.42 | Woodstown Rd |  Tomlin Station Rd |
Tomlin Station Rd |
|
| 14.28 | Woodstown Rd |  Swedesboro-Monroeville Rd |
Swedesboro-Monroeville Rd |
|
| 13.65 | Woodstown Rd | Branch Oldmans Creek | culvert | Branch Oldmans Creek |
| 13.53 | Woodstown Rd |  Swedesboro-Harrisonville Rd |
Swedesboro-Harrisonville Rd |
|
| 12.58 | Woodstown Rd | Branch Oldmans Creek | culvert | Branch Oldmans Creek |
| 12.49 | |
South Harrison Twp, Gloucester Co Pilesgrove Twp, Salem Co |
||
| 12.49 | |
Oldmans Creek | bridge | Oldmans Creek |
| 11.96 | Mullica Hill Rd | Branch Oldmans Creek | culvert | Branch Oldmans Creek |
| 11.82 | Mullica Hill Rd | Kettles Run | culvert | Kettles Run |
| 11.19 | Mullica Hill Rd |  Point Airy Rd |
Point Airy Rd |
|
| 10.45 | |
Pilesgrove Twp, Salem Co Woodstown Boro, Salem Co |
||
| 10.33 | Main St | Bypass Rd |
Elridges Hill Rd |
|
| 10.14 | Main St | West Jersey Railroad | overpass | West Jersey Railroad |
| 9.90 | Main St |  Auburn St |
||
| 9.88 | Main St | Elm St |
||
| 9.65 | Main St |  Marlton Rd |
||
| 9.44 | Main St Salem Rd |
|
East Ave Main St  |
|
| 9.18 | Salem Rd | Salem Creek | culvert | Salem Creek |
| 8.98 | Salem Rd | Woodstown Boro, Salem Co Pilesgrove Twp, Salem Co |
||
| 8.80 | Salem Rd | Harding Hwy |
|
Bailey St |
| 8.04 | Salem Rd | Nichomus Run | culvert | Nichomus Run |
| 7.38 | Salem Rd | Cemetery Rd |
||
| 7.05 | |
Pilesgrove Twp, Salem Co Mannington Twp, Salem Co |
||
| 7.05 | |
Majors Run | culvert | Majors Run |
| 6.72 | |
 Haines Neck Rd |
||
| 6.68 | |
Cheney Rd |
||
| 5.21 | |
Colliers Run | bridge | Colliers Run |
| 4.11 | |
Mannington Creek | bridge | Mannington Creek |
| 3.98 | |
Acton Station Rd |
||
| 3.28 | |
Welchville Rd |
||
| 2.12 | Kings Hwy | Pointers-Auburn Rd |
||
| 0.43 | |
Mannington Twp, Salem Co Salem City, Salem Co |
||
| 0.43 | |
Fenwick Creek | bridge | Fenwick Creek |
| 0.16 | Market St |  Griffith St |
|
Grant St |
| 0.00 | Market St |  Broadway |
|
Broadway |
| 0.00 | Southern terminus of , Salem City, Salem Co |
|||
| Road continues southward as Market St | ||||